杂交一代(尼罗罗非鱼♀×奥利亚罗非鱼♂)及其亲本基因组DNA的比较

Comparison of genomic DNA of hybrid ( Oreochromis niloticus ♀ × O.aureaus ♂) and its parents

采用RAPD技术 ,用 2 0个随机引物对尼罗罗非鱼、奥利亚罗非鱼及其杂交一代的精巢DNA进行扩增反应 ,发现引物OPZ - 14和OPZ - 18在尼罗罗非鱼和奥利亚罗非鱼之间扩增出有差异的DNA片段 ,作为鉴定两种鱼的分子标记有较高可信度。对这两种鱼的杂交一代基因组DNA的RAPD扩增能获得足够的多态性 ,2 0个引物中有 8个引物扩增出差异性产物 ,表明杂交一代基因杂合性增强 ,这是杂种优势得以形成的重要遗传物质基础之一。

Using Random Amplified Polymorphic DNA(RAPD) technique with 20 kinds of random primers, genomic DNA of hybrid (Oreochromis niloticus ♀ ×Oreochromis aureaus ♂) and its parents were analysed. The results showed that only with the two kinds of primers, OPZ-14 and OPZ-18, species specific fragments were produced between parent fishes, even between different fish belonging to the same species, and some of them could be used as molecular markers to identify O.niloticus and O.aureaus. And 8 primers generate polymorphic products among hybrid fish and its parents. DNA fragments amplified from the hybrid fish were more abundant than those from its parents, which may be the result of more abundant genes in the hybrid fish, because of heredity or mutation. These results provide some molecular biological basis for species identification and heterosis effect in fish.