增加贝类染色体分裂相的方法初探

On Methods of Increasing the Metaphase of Shellfish

研究以太平洋牡蛎为材料 ,用低温同步化法和 8μg/m L的植物血球凝集素 (PHA- M)、50μg/m L 伴刀豆蛋白 A(Con A)两种外源凝集素分别处理其鳃 ,后用鳃做染色体滴片 ,10 % Giemsa染色后镜检 ,统计分裂相数。发现三种方法都能使分裂相比例增加 ,其中低温同步化法效果最好 ,可使分裂相比例由对照的 0 .7× 10 -4 升高到 6 .5× 10 -4 ,提高 8.3倍。PHA- M法使分裂相比例由对照的 0 .7× 10 -4升高到 5.5× 10 -4 ,提高 6 .8倍 ,Con A法使分裂相比例由对照的 0 .7× 10 -4升高到3.6× 10 -4 ,提高 4 .1倍。

Three methods, low temperature synchronizing and two lectins——8μg/mL PHA-M and 50μg/mL ConA, were used to treat the gill of Pacific oyster (Crassostrea gigas). The gill was dropped on a slide, stained with 10% Giemsa and then examined with microscope for the metaphase to be counted. The proportion of metaphase increased after the gill was treated by all the three methods. We find that low temperature synchronizing is the best one. It can increase the proportion from 0.7×10 -4 to 6.5×10 -4, which means an increase of 8.3 times. PHA-M treatment can increase the proportion from 0.7×10 -4 to 5.5×10 -4, the increase is 7.8 times, and ConA treatment can increase the proportion from 0.7×10 -4 to 3.6×10 -4, which is 4.1 times.