透明体的光学定量测量

Quantitative measurement of transparent object

综述了使用光学方法测量透明体的方法。介绍了两种常用的光学方法——相衬法和微分干涉相衬法。在 2 0世纪 80年代 ,一般认为相衬法和微分干涉相衬法只适用于定性观察而不能实现定量测量 ,主要是因为在其成像原理中的非线性因素的影响。但是对于微分干涉相衬法 ,最近国内外出现了几种定量测量的方法 ,本文对这几种方法进行了总结和比较。

In the paper, the methods of optical measurement of transparent object are introduced The two most widely used techniques for optical phase contrast microscopy are the Zernike and the Normaski methods In the 1980s, it is thought that the two methods are only used for qualitative analysis, can not be directly applied in optical metrology The main reason is the effect of nonlinear factors in the imaging principle But for DIC methods, there are some quantitative measurement methods