BT型细胞质雄性不育恢复基因的基因定位

Molecular Location of a Fertility Restorer Gene for BT Type CMS Rice

以典败率、圆败率、染败率、花粉育性和小穗育性为育性指标 ,对 BT型细胞质雄性不育系 731A、恢复系 C90 83以及 731A/C90 83的 F1 、731A//731B/C90 83的三交 F1 等亲本和杂种群体的育性进行调查分析 ,并以 731A//731B/C90 83的三交 F1 群体为材料进行 RFL P和微卫星标记分析 ,进行基因定位。结果表明 ,C90 83具有 1个主效的显性恢复基因。选用第10染色体上的 7个 RFL P和微卫星标记分析两个亲本 ,有 3个 RFL P标记在两个亲本间有多态 ;选用其中的 C16和 G2 91分析 731A//731B/C90 83的三交 F1 中的各个单株的结果表明 ,这两个 RFLP标记与 C90 83的恢复基因连锁 ,C16与这个恢复基因之间的交换值为 19.3% ,G2 91与这个恢复基因之间的交换值是 14.0 % ,C90 83的恢复基因与已报道的 BT型细胞质雄性不育恢复基因

Inheritance of fertility restoration of BT type CMS was conducted by utilizing a CMS line 731A (A), a CMS maintainer line 731B(B), a CMS restorer line C9083(C) and their (A/C)F 1 and (A//B/C)BC 1 populations. The results indicated that fertility restoration of C9083 was conditioned by one dominant gene; the parents C9083 and 731A, and their populations (A//B/C)BC 1 were also used as plant materials to map this gene with RFLP and microsatellite markers on chromosome 10.This gene was linked with RFLP markers C16 and G291, with recombinant value between the gene and C16 being 19.3% and that between the gene and G291 being 14.0%, therefore the gene might be allelic to the Rf1 gene reported before.