8株鳖源变形杆菌外膜蛋白的比较

Comparison of outer membrane protein patterns of 8 Proteus strains of soft shelled turtle origin

从 1 2批送检病鳖体内分离出 8株细菌 ,经形态学检查、生理生化特性测定、致病性测定和血清学鉴定 ,确定 7株为普通变形杆菌 ,1株为奇异变形杆菌。进一步采用十二烷基硫酸钠 (SDS)破菌法提取 8株鳖源变形杆菌的外膜蛋白 (OMP)进行SDS PAGE电泳 ,比较分析细菌OMP型。结果显示奇异变形杆菌的OMP型由 3条相对分子质量范围为 3 0× 1 0 4～4 3× 1 0 4的主要蛋白带组成 ;7株普通变形杆菌的主要外膜蛋白相对分子质量范围为 3 0× 1 0 4～6 7× 1 0 4,分属 2个OMP型 ,其中 4个分离株属OMP1型 ,由 4条主要蛋白带组成 ;其余 3个分离株属OMP2型 ,由 7条主要蛋白带组成。表明不同种变形杆菌的OMP型差异较大 ,同种不同株变形杆菌的OMP型相似 ,但蛋白带的迁移率及颜色深浅在菌株间仍有差异。此外 ,发现相对分子质量约为 4 3× 1 0 4的一条外膜蛋白带为所有菌株所共有 。

The outer membrane proteins(OMPs)of 8 Proteus strains isolated from pathogenic soft shelled turtles were extracted with SDS chemical method and were compared by SDS PAGE.The results showed that the OMP pattern of P.mirabilis was made of three protein bands with relative molecular weight ranging from 3 0×10 4～4 3×10 4.Two OMP patterns were divided into 7 P.vulgaris strains.OMP1 patten made of four protein bands was shared by 4 P.vulgaris strains;OMP2 pattern made of seven protein bands was shared by 3 P.vulgaris strains.These results indicated that OMP patterns had significant differences between P.mirabilis and P.vulgaris but they were similar among different strains of P.vulgaris.The protein band with 4 3×10 4 relative molecular weight was found in all strains,which might be common antigen of Proteus.