摘要
根据马立克氏病病毒 (MDV)国际标准株 GA的基因序列 ,设计合成一对引物。通过 PCR技术 ,分别以弱毒疫苗株 81 4和广西分离强毒株 G2 株基因组为模板 ,扩增获得预期大小的 PCR产物 ,将 PCR产物和 p UC1 8分别经 Bam H 和 Sma 酶切后 ,连接、转化 TG1 ,筛选获得了 81 4 -g I和 G2 -g I的基因克隆 ,将所得克隆进行测序 ,并将它们与已发表的MDV其他毒株 g I的序列进行比较分析 ,获得了它们的同源性 。
Glycoprotein I (gI) genes were amplified from genomic DNA of MDV 814 and G 2 strains by PCR. The amplified fragments were cloned into pUC18 vector plasmid via restriction endonuclease Bam HⅠ and Sma Ⅰ repectively. The recombinants were screened by restriction endonucleas analysis. The obtained positive gI genes marked 814 gI and G 2 gI were sequenced and compared with the other reported gI sequences of MDV. The homology of gI for different MDV strains was analysised and the phylogentic tree was also drawed.
基金
国家自然科学基金资助项目 !(3 9870 0 0 8)
