摘要
本文报道了特定电磁波(TDP)处理对水稻、小麦、玉米、番茄等植物种子吸胀、萌发过程中及其幼芽、幼苗中超氧化物歧化酶(SOD)活力及其同工酶的影响,电磁波长为0.25～50μm,处理时间为1～5 h 不等,处理温度控制在30±2℃,SOD 活力测定采用酶抑制氯化硝基四唑兰(NBT)光还原法。试验结果表明,0.25～50μm的电磁波明显提高了上述植物中 SOD 活性,增加幅度一般在11%～100%之间,试验结果亦指出,特定电磁波未改变 SOD 同工酶酶谱。作者认为 TDP 对 SOD 活力的提高有助于提高植物的抗逆性能力。
Superoxide Dismutase is very important in plant system.The enz- yme,dismutating superoxide free radical(.O_22^-)to molecular orygen and H_2O_2, plays an essential role in defensing against potential toxicity of oxygen.It also protects membrane from lipid preoxidation.In a word,the enzyme is highly related to the resistance to atress.We studied the effect of irradiation with TDP on the activity and isoenzyme spectra of SOD in several plants. Irradiation apparently raised the enzyme activity,but no elect on the numbers of isoenzyme spectra at early developing stage.
基金
国家自然科学基金
关键词
特定电磁波
超氧化物歧化酶
植物
specified electromagnetic wave
superoxide dismutase
polyacrylamide gel electrophoresis
