LR-98对肝癌细胞增殖的抑制性效应

Inhibitory Effects of LR-98 on Proliferation of Hepatocarcinoma Cells

利用在体和离体实验相结合 ,以动物接种肿瘤细胞后的生存时间、瘤重与体重比值、肝癌细胞生存率、超氧化物歧化酶 (SOD)活性及肝癌细胞凋亡为指标 ,研究了不同剂量榄仁树叶提取物 (LR 98)对肝癌细胞增殖的抑制性效应及可能机制 .在体实验结果表明 ,5× 10 - 3g/mLLR 98处理组动物的生存时间较对照组明显延长 (P <0 .0 1) ,不同剂量处理组 (5× 10 - 3g/mL ,2× 10 - 2 g/mL ,5× 10 - 2 g/mL)的瘤重与体重比值均较对照组明显减小 (P <0 .0 5) .体外培养的细胞经不同剂量LR 98(1× 10 - 5g/mL ,5× 10 - 5g/mL ,1× 10 - 4 g/mL)作用后 ,生存率及SOD活性均显著下降 (P <0 .0 1) .流式细胞仪分析表明 ,不同剂量LR 98作用于肝癌细胞 8h后 ,在G1 期前均出现一亚二倍体峰 ,表明均能诱导肝癌细胞发生凋亡 ,凋亡率分别达 2 9.4 4 % ,2 4 .4 5%和 2 5.2 2 % .以上结果说明LR 98可显著抑制肝癌细胞增殖 .降低SOD活性 ,诱导肝癌细胞凋亡可能是LR 98抑制肝癌细胞生长的机理之一 .

The following treatment of different doses of the extract of leaves of Terminalia catappa (LR 98), the survival days and the values of tumor weight/body weight of animals vaccinated with hepatocarcinoma cells were observed, and the measurements of cell viability and superoxide dismatase (SOD) activity were carried out in hepatocarcinoma cell cultures to study the hepatoprotective effects of LR 98 and the possible mechanisms. Meanwhile, apoptosis of hepatocarcinoma cells was detected by flow cytomety (FCM) . The experiment in vivo showed that the survival days were obviously extended, and the values of tumor weight/body weight were markedly reduced by the oral administration of LR 98 (5×10 -3 ?g/mL,2×10 -2 ?g/mL, 5×10 -2 ?g/mL) . Of cultured hepatocarcinoma cells, both cell viability and SOD activity were significantly decreased after treatment of LR 98 (1×10 -5 ?g/mL,5×10 -5 ?g/mL,1×10 -4 ?g/mL).The analysis with FCM showed: 'Sub G 1' peak occurred in DNA histogram of FCM at 8 h following the addition of LR 98, which indicated that proliferation of hepatocarcinoma cells was inhibited and apoptosis was induced. The percentage of apoptotic cells in culture was 29.40%,24.45%,25.45% respectively when treated with different concentration of LR 98 (1×10 -5 ?g/mL,5×10 -5 ?g/mL,1×10 -4 ?g/mL). The experimental data indicated that LR 98 could inhibit the proliferation of hepatocarcinoma cells. Mechanisms underlying its antitumor effect may be related to decrease in SOD activity and induction of apoptosis of tumor cells.