四株肺癌细胞系的维甲酸受体表达的研究

A comparative study of the expression of retinoic acid receptors (RARs) in the four cell lines of human pulmonary carcinoma

目的 :观察来源于不同组织学类型的人肺癌细胞系 (肺巨细胞癌细胞系PLA - 80 1,肺鳞状上皮细胞癌细胞系C - 57,个旧肺腺癌细胞系GLC - 82和肺腺癌细胞系PC - 84 0 4 5)的维甲酸受体的含量和分布特征 方法 :作ELISA和免疫组化显色计算机图象分析方法 ,对 4株具有不同侵袭潜能 ,不同组织学类型的肺癌细胞系的维甲酸受体 (RARs、RAR和RXRs)进行检测 结果 :4株细胞系的RARβ的表达极少 ,甚至缺如 ;RARs主要分布在癌细胞胞浆中 ,但RXRs主要位于细胞核内 ,表达水平较高 ;RARs和RXRs在PLA - 80 1细胞中表达最低 结论 :肺癌中普遍存在着RAR的表达分布异常 ,这些改变可能与肺癌的异常分化有关 ,亦可能是肺癌细胞对维甲酸诱导分化不敏感的重要原因 RXRs较高水平的表达且主要位于核内 ,提示用

Aim:To observe the expression and distributive characteristics of RARs in the four cell lines of human pulmonary carcinoma including pulmonary large cell carcinoma cell line PLA-801, pulmonary squamous cell carcinoma cell line C-57, Gejiu lung adenocarcinoma cell line GLC-82 and pulmonary adenocarcinoma cell line PC-84045, which originated from different histologic type. Methods:The analyses of RARs, RARβ and RXRs were conducted by ELISA and immunohistochemistry technics combining computer image analysis. Results:The results showed: (1)the level of RARβ in all four cell lines was very low, even lost, (2) RARs was mainly located in the cell plasma of the four cell lines, but RXRs mainly in the cell nucleus and the level of its expression was relatively higher, (3) the level of RARs and RXRs in the PLA-801 cell line was the lowest. Conclusion:The loss of RARβ and the abnormal location of RARs existed commonly in the lung carcinoma cell, and was closely related to abnormal differentiation of pulmonary carcinoma, and probably was an important cause uninfluenced to all-tran-retinoid acid (AT-RA), and the effect of inducing differentiation with 9-cis-retinoic acid might be better than that with AT-RA. [