一个新的靶点的反义bcl-2寡核苷酸诱导HL-60细胞凋亡的研究

Induction of apoptosis in HL-60 cells by a new antisense oligodeoxynucleotide targeting for the bcl-2 coding sequences

目的 :探讨针对bcl- 2mRNA蛋白编码区的反义寡核苷酸对HL - 60细胞bcl - 2蛋白表达和凋亡的作用 方法 :应用台盼蓝拒染法和流式细胞仪检测HL - 60细胞的活性和bcl- 2蛋白表达和细胞凋亡的情况 结果 :10 μmol/L和 2 0 μmol/L的反义寡核苷酸能抑制HL - 60细胞bcl- 2蛋白表达和诱导细胞凋亡 ,并且针对bcl - 2mRNA蛋白编码区的反义寡核苷酸比针对bcl - 2mRNA翻译起始区的反义寡核苷酸作用更强 结论 :针对bcl- 2mRNA蛋白编码区的反义寡核苷酸能抑制HL - 60细胞bcl-

Aim: To observe the effect of bcl-2 antisense oligodeoxynucleotide targeting the coding region of the bcl-2 mRNA on the expression of bcl-2 protein and apoptosis in HL-60 cells. Methods: Cell viability、the expression of bcl-2 protein and apototic cells were observed by propidium iodide exclusion and flow cytometric. Results: The bcl-2 antisense oligodeoxynucleotide of 10 μmol/L and 20 μmol/L can inhibit bcl-2 protein expression and promote apoptosis of HL-60 cells , and the antisense oligodeoxynucleotide targeting the coding region of the bcl-2 mRNA has stronger action than that targeting the transtration initiation. Conclusion: The bcl-2 antisense oligodeoxynucleotide targeting the coding region of the bcl-2 mRNA can inhibit bcl-2 protein expression and promote apoptosis of HL-60 cells.[