摘要
用改良的冷酚法从大鼠心房中提取总RNA,经紫外分光光谱、变性琼脂糖凝胶电泳及分子杂交放射自显影检测表明,RNA制备物纯度高、未降解。此方法简便不需特殊试剂和设备。
The RNA was extracted with the improved cold phenol method from rat atria. Several examinations, including ultraviolet spectrophotometric examination and electrophoresis, run in denatured agarose gel and molecular hybridization for RNA samples showed that RNA preparations had satisfactory purity and undegradation. This extraction procedure is very simple and does not need any special reagents and equipments.
出处
《白求恩医科大学学报》
CSCD
1992年第2期105-107,共3页
Journal of Norman Bethune University of Medical Science
基金
国家自然科学基金
关键词
RNA
分子杂交
分离制备
RNA Molecular hybridization Separation and preparation
