人参二醇组皂甙对休克细胞的保护作用及其机理的实验研究

Protective Effect of Panaxadiol saponin on Cells in Hemorrhagic Shock and its Mechanism

29只杂种犬,随机分为失血性休克人参二醇组皂甙组(HSP)和失血性休克组(HS)。结果发现人参二醇组皂甙有如下作用:①抑制细胞内酶的释放,失血后第5h时HSP组血清GOT、GPT、LDH和CK水平显著地低于HS组(P<0.05);②对心肌细胞、肺泡Ⅱ型上皮细胞及血小板的超微结构具有明显的保护作用;③通过降低血清去甲肾上腺素含量的过度增高,改善微循环,减轻酸中毒。激活SOD,减少过氧化脂质产生而保护细胞与亚细胞结构。

Twenty-nine mongrel dogs were randomly divided into 2 groups: (1) HSP group, (hemorrhagic shock pretreated with Panaxadiol saponin group); (2) HS group (hemorrhagic shock pretreated with the same volume of saline). The experimental results indicated that; (1) serum GOT, GPT, LDH and CK levels in the HSP group was significantly lower than that of the HS group (P<0. 05) at the 5th hour; (2) severe damage of the ultrastructures of type Ⅱalveolar epithelial cells and myocadial cells in the HS group was observed; however all changes in the HSP group were comparatively mild; (3) serum [H+] of the HSP group was obviously lower than that of the HS group (P<0. 05) ; (4) serum lipid peroxide level of the HSP group was significantly lower than that of the HS group (p<0. 05) at the 5th hour; (5) serum superoxide dismutase (SOD) activity of the HSP group was obviously higher than that in HS group (p<0. 05); (6) serum ME content in the HSP group was significantly lower than that of the HS group.