实验性糖尿病大鼠心肌细胞膜通透性的改变

ALTERATION OF SARCOLEMMAL MEMBRANEPERMEABILITY OF CARDIAC MUSCLE CELLSIN EXPERIMENTAL DIABETIC RATS

用辣根过氧化物酶(HRP)作示踪物研究链脲佐菌素引起的8周实验性糖尿病大鼠心肌细胞膜通透性的变化。结果表明,糖尿病大鼠心肌中HRP阳性肌细胞数明显多于对照动物。这一结果提示,糖尿病大鼠心肌细胞膜通透性明显增大。这种异常的细胞膜通透性增大在糖尿病性心肌病的发生机理中可能起重要作用。

Alteration of sarcolemmal membrane permeability of cardiac muscle cells in strep-tozotocin-induced diabetic rats was examined with horseradish peroxidase (HRP) as a tracer. Wistar rats were made diabetic by streptozotocin injection (i. p., 70mg/kg b. w.). Age-matched, untreated rats served as the controls. After 8 weeks of diabetes, HRP (10mg/100g b. w.) was injected intravenously and its localization in the ventricular myocardium was examined with light microscopy. The experimental results showed that the percentage of HRP-positive cardiac muscle cells in the heart of diabetic rats was significantly greater as compared with the age-matched controls, indicating that sarcolemmal membrane permeability of cardiac muscle cells in diabetic rats was markedly increased. The present study suggests that abnormal membrane permeability of cardiocytes may play an important role in the pathogenesis of diabetic cardiomyopathy.