急性胆道感染时肝腺泡酸性磷酸酶与细胞色素氧化酶活性的变化

Changes of acid phosphatase and cytochrome oxidase in hepatic acini during acute obstructive cholangitis in rats

本文从肝腺泡角度出发,应用酶组化染色方法,结合图像分析技术,客观定量测定急性胆道感染时肝腺泡不同区带肝细胞ACP、CO活性变化。发现:胆道感染时,ACP有一个先激活后抑制过程,其中以第1带肝细胞变化最显著;而CO在感染早期其活性已发生抑制,随病变发展进行性加重,主要以第3带肝细胞变化明显。表明肝腺泡不同区带肝细胞在胆道感染时对致病因素的反应不尽相同。

The changes of acid phosphatase(ACP)and cytochrome oxidase(CO)in the hepatic acini were investigated with image analysis in 90 Wistar rats after acute obstructive cholangitis was inflicted.It was found that the activity of ACP was significantly increased in the 2nd hour after surgery and markedly decreased in the 12th hour and these changes were predominantly located in acinur zone 1 of the hepatocytes.The activity of CO was decreased progressively after surgery,and these changes were quite marked in the acinur zone 3 of the hepatocytes.These findings indicate that the reactions towards acute obstructive cholangitis are different in the different acinur zones of the hepatocytes.