大肠癌“移行粘膜”病理变化研究——粘液组化、免疫组化及电镜观察

Mucohistochemical, immunohistochemical and electron microscopic study on the pathological changes of "transitional mu-cosa" of colorectal carcinoma

对200例大肠癌旁“移行粘膜”进行了粘液组化HID/AB及PAT/KOH/PAS检测,单克隆抗体MC_3和CEA免疫组化标记,凝集素PNA、UEA-1及DBA受体测定,扫描电镜观察。其结果与10例正常直肠粘膜、130例癌组织、155例各型腺瘤型息肉及3～5个月胎儿大肠粘膜进行对照。结果表明大肠粘膜从良性到恶性的各阶段粘液组化及免疫组化的改变有显著差异(P<0.05或P<0.01),癌旁“移行粘膜”也存在类似癌及胎儿大肠的不成熟性病理变化。异常范围88.0％的病例在2～3cm以内。

Two hundred specimens of the 'transitional mucosa' of colorectal carcinoma were studied with mucohistochemical methods of HID/AB and PAT/KOH/PAS,immunohistochemical methods of MC3,CEA,lectin receptor PNA,UEA-1 and DBA,and scanning electron microscopy.10 specimens of normal colonic mucosa.130 specimens of colorectal carcinoma,155 specimens of various types of colorectal adenoma and polyp,and 24 sepcimens of normal colonic mucosa of fetus aged 3 to 5 months were also examined to serve as controls.It was found that in every stage of the malignant deterioration of the colorectal mucosa,there was significant difference between the mucohistochemical changes and the immunohistochemical changes(P<0.05 or P<0.01),and there were immature tissues in the juxtacancerous transitional mucosa which were similar to those seen in carcinoma and fetal colonic mucosa.In 88.0% of colorectal carcinoma cases,these precancerous changes were found in a zone 2 ~ 3cm in width around a cancerous lesion.