二氧化硫体内衍生物诱发中国仓鼠肺细胞微核的效应

Inductive Effects of Intravital Derivatives of Sulfur Dioxide on Frequencies of Micronucleus of Lung Fibroblast Cells in Chinese Hamster

目的 为了解二氧化硫 (SO2 )对哺乳类细胞的遗传毒理效应。方法 以 SO2 在体内的衍生物——亚硫酸氢钠和亚硫酸钠处理培养的中国仓鼠肺成纤维细胞 (CHL) ,并对其诱发微核 (MN)形成的作用进行了分析。结果 对照盐水组和 1、2、5、10 mmol/ L 的 SO2 衍生物处理组 CHL的 MN率 (‰ )分别为 8.17、9.83、10 .33、12 .83、13.33及 14.17,从2 mmol/ L的 SO2 衍生物处理组开始与对照组差异有显著性 ,且呈剂量依赖关系。无论是否有 S9混合物存在 ,SO2 衍生物均可诱发 CHL 的 MN率显著增高 ,且 SO2 衍生物处理细胞时间愈长 ,引起细胞遗传损伤所需的最低浓度就愈低。结论 SO2 是不需要体内酶促转化的直接的细胞染色体断裂剂和基因毒性因子。同时也启示 ,长期接触环境低浓度 SO2 污染 ,有引起人体细胞遗传物质损伤的潜在危险。

Objective To understand the cytogenetic toxicological effects of sulfur dioxide on mammalian cells Methods The micronulei of lung fibroblast cells (CHL) in Chinese hamster treated by intravital derivatives of sulfur dioxide,sodium hydrosulfite and sodium sulfite,were observed and analysed Results The frequencies of micronucleus of control group of CHL exposed to physiological saline and exposure groups exposed to intravital derivatives of sulfur dioxide at concentrations of 1,2,5,10 and 15 mmol/L were 8 17‰,9 83‰,10 33‰,12 83‰,13 33‰ and 14 17‰ respectively The frequencies of micronucleus of exposure groups revealed and dose dependent relationship and significant differences compared with those of control group except group exposed to 1 mmol/L derivatives of sulfur dioxide The significant increase of frequencies of micronucleus in CHL cells could be induced by derivatives of sulfur dioxide whether the S 9 mixture was added or not The logner the treatment period of CHL cells with the derivatives of sulfur dioxide was,the lower the minimum concentration required for inducing the genetic injury of CHL cells was Conclusion Sulfur dioxide was a direct chromosomal breakage agent and gene toxicity factor to mammalian cells without the catalysis of intravital enzyme Long term exposure to low levels of sulfur dioxide in environment might be a potential risk to injure human cytogenetic materials