膀胱移行细胞癌组织端粒酶活性和增殖细胞核抗原的表达及意义

The clinical significance of telomerase activity and PCNA expression in transitional cell carcinoma of the urinary bladder

目的 探讨膀胱移行细胞癌 (TCCB)组织中端粒酶活性和增殖细胞核抗原 (PCNA)的表达关系及临床意义 方法 采用TRAP ELISA法及免疫组化方法测定 5 8例TCCB组织的端粒酶活性和PCNA表达情况。 结果 TCCB的端粒酶阳性率为 86 .2 % (5 0 / 5 8) ,不同病理分级或临床分期之间差别无显著性意义 (P >0 .0 5 ) ;PCNA表达强度与分级或分期呈正相关 (P <0 .0 5 ) ;不同PC NA表达强度的TCCB样品之间 ,端粒酶活性差别有显著性意义 (P <0 .0 5 ) ,二者呈正相关。 结论 TCCB组织中端粒酶活性与PCNA表达有较好的协同性 ,二者联合检测对TCCB的诊断治疗和估计预后有重要意义。

Objective To study the clinical significance of telomerase activity and the proliferating cell nuclear antigen (PCNA) in transitional cell carcinoma of urinry bladder (TCCB) Methods Telomerase activity was examined by TRAP-ELISA and PCNA expression by immunohistochemistry in 58 cases of TCCB. Results 50 of 58 cases (86.2%) exhibited telomerase activity.No significant diffence of telomerase activity positive rate was observed in tumors of different grade and stage.Whereas,PCNA expression had a significant association with tumor grade and tumor stage (P<0.05).A significant positive relationship was observed between telomerase activity level and PCNA expression (P<0.05). Conclusions There is a good correlation between telomerase activity level and PCNA expression and therefore the two examined at the same time might be helpful in the diagnosis,treatment and prognostic evaluation of TCCB.Of the above two,telomerase perhaps plays the more important role in maintaining stability of chromosomes in TCCB.