TGF-β_1对人前列腺上皮细胞增殖与凋亡的调节作用

The regulatory effect of transforming growth factor-β_1 on the proliferation and apoptosis of human prostatic epithelial cells

目的 观察TGF β1对体外培养人前列腺上皮细胞增殖与凋亡的调节作用。 方法 建立原代人前列腺上皮细胞株 13例 ,角化蛋白、PSA等免疫组织化学染色鉴定培养细胞 ,绘制细胞生长曲线、运用BrdU方法和细胞凋亡原位染色观察传代后细胞生长状况及不同剂量TGF β1对细胞增殖与凋亡的作用。 结果 培养细胞角化蛋白、PSA染色阳性。TGF β1抑制细胞增殖并诱导细胞凋亡 ,作用与剂量成正比 ,EGF与TGF β1相互减弱对方的作用。 结论 TGF与TGF β1对前列腺上皮细胞增殖与凋亡状态起重要的调节作用。

Objective To investigate the regulatory effect of transforming growth factor-β 1 (TGF-β 1)on the proliferation and apoptosis of human prostatic epithelial cells in vitro. Methods The primary culture was prepared by collagenase dissociation of minced prostatic tissue obtained from 8 BPH patients (53 to 70 of age) on open prostatectomy and from 5 organ donors (23 to 36).Cells were cultured in serum free prostate epithelial cell growth media and identified by immunocytochemical studies.The cells were passaged and cultured in media free of bovine pituitary extract and EGF and TGF-β 1 and EGF in different doses were then added either alone or in combination according to experimental requirement.The rate of cell proliferation was assessed by counting the cell number and by 5-bromo-2'-deoxyuridine (ELISA).Apoptosis induced by TGF-β 1 was examined by in situ transferase-mediated dUTP nick and labeling. Results The cultured cells had the typical morphological features of epithelium and stained positive for PSA and Pan-Keratin.The cells from normal prostates of young donors showed more mitogenic activity than those from BPH prostates.EGF enhanced cell proliferation whereas TGF-β 1 inhibited cell proliferation which could be reversed by increasing concentration of EGF.TGF-β 1 induced apoptosis of human prostatic epithelial cells.The effect of TGF-β 1 and EGF was concentration dependent. Conclusions TGF-β 1 is a direct physiological regulator of cell proliferation and cell apoptosis of human prostatic epithelial cells.