颈髓损伤外周血淋巴细胞的表达及临床意义

The Changes of Peripheral Blood Lymphcyte Subuts in the Patients with Cervical Cord Injuries and their Clinical Significances

目的 探讨颈髓损伤 (cervicalcordinjuries ,CCI)外周血淋巴细胞表达的相互作用及临床意义。方法 用免疫荧光标记流式细胞仪检测了 2 0例急性颈髓损伤患者外周血淋巴细胞CD19、CD3、CD4 、CD8、CD69、CD54 、CD56表达的变化 ,并与正常人群进行比较。结果 T细胞CD3、CD4 和CD4 /CD8比值显著降低 (P <0 0 1) ,而早期活化标志CD69则显著升高 (P <0 0 1)。B细胞CD19表达明显增加 ,粘附分子CD56和CD54 表达也明显增加 (P <0 0 1)。结论 以上结果提示急性颈髓损伤后机体细胞免疫功能发生明显变化 ,主要表现为T细胞的减少 ,B细胞的增加以及淋巴细胞活化抗原和粘附分子的表达增加。CCI对细胞功能造成严重损伤 。

Objective To investigate the effect of the CCI in the peripheral blood lymphcyet (PBL) subsets in patients with acute cervical cord injuries( CCI) Methods The PBL subsets were determined by immunofluorense labelling and flow cytometry in 20 cases of acute cervical cord injuries, and the data were compared with those in the healthy donors Results It was found that the CD 3 , CD 4 cells and the CD 4/CD 8 ratio were significantly lower(P<0 01) in the CCI patients than those in the controls , and the CD 19 , CD 69 ,CD 56 and CD 54 cells were much higher (P<0 01) in the CCI patients Conclusion The immune status of patients with acute cervical cord injuries were somewhat depressed, intercellular adhesion molecules played some role in the acute cervical cord injuries