高效液相色谱法测定分娩产妇血清中安定的浓度及药动学研究

Determination of Serum Concentrations of Diazepam in Parturients and Its Pharmacokinetic Study

本文建立了反相高效液相色谱法测定安定的血药浓度。柱Ｃ１８，流动相为甲醇－水（７１：２９），水中含０．１４ｍｌ正丁胺和０．０９ｍｌ冰醋酸）；检测波长２３０ｎｍ。在０．１～３μｇ／ｍｌ浓度范围内线性良好，最低检测限为５０ｎｇ／ｍｌ。平均回收率为８８．１９±０．５％。用本法测定了分娩产妇ｉｖ注安定后的体内血药浓度，并进行了药代动力学参数计算。

A Rp-HPLC Method has bee developed for the determination of diazepam(DZP) in serum.Chromatographic separation has been achieved on C18 column with methanol-water (71:29, water contains 0.14ml n-butylamine and 0.09ml acetic acid glacial) as the mobile phase. Detection was set at UV 230nm. The lower limit of detection of DZP in serum was 50ng/ml. The calibration curve was linear between 0.1 and 3μg/ml with a regssion coefficient of 0.9988. This assay procedure has been applied to monitoring serum levels of diazepum in parturients who received IV administration of drug.The pharmacokinetics of diazepum could be described by a two compartment open model.Its parameters were as follows:t1/2=4.87±2.09min,t1/2=184.72±36.59min,AUC=151.05±42.20μg/ml/min.