三种调味品的果蝇伴性隐性致死试验

THE THREE FLAVOURINGS IN THE SEX-LINKED RECESSIVE LETHAL TEST OF DROSOPHILA MELANGOASTER

桂皮、茴香和花椒是人们普遍喜爱的调味品。为了对它们的安全性作出精确的评价,我们用黑腹果蝇(Drosophla melanogaster)的野生型原种Oregon K品系和Basc(Muller-5)品系作为材料,从其F-2检查了阴性对照、阳性对照和三种调味品对它们生殖细胞三个发育阶段(成熟精子、精细胞和精母细胞)的伴性隐性致死(SLRL)的诱变性。三种调味品均以模拟实际烹煮的方式提取,其浓度为(250mg/ml)。经用Kastenbaum-Bowman方法对所有测试数据进行统计分析,结果表明为MMS阳性对照组与所有测试组比较时,其SLRL的诱变率均远远超过正常对照组(CK)的二倍,差异显著。根据资料,Oregon K品系的SLRL自发突变率多年来都稳定在0.4％。而所有测试组包括阴性对照组的SLRL诱变率均未超过此界限,因此可以认为这三种调味品均不具有SLRL诱变性。

Since Cinnamomum japonicum, Illicium verum and Zanthoxylum bungeanum are People's favourite, it seems to be necessary to evaluate their safety accurately. Wild-type Oregon K strain of Drosophila melanogaster and Mutant stock of Base (Muller-5) were used in the test. From F2 generation the mutagenicity had been detected in three stages of germ-cell development by sex-linked recessive lethal test of Drosophila in negative control, positive control and the three flavourings treated. Concentration 250mg/ml of the original solutions of the three flavourings was used. We performed and analysed all datum by Kastenbaum-Bowman test. The results show that when the positive control of MMS is compared with all treated groups, the mutation frequency of SLRL is much more than two times of treated groups and their difference is significant. The mutation frequencies of SLRL in all of the treated groups in cludirg negative control are below the limit of the spontaneous mutation rate of Oregon K strain, which is scored stably 0.4% for several years. So the three flavourings have no detectable mutagenecity ofSLRL. We are now doing short-term genotoxicity assays by mice to further test this result.