常用抗癌药物诱发人外周血淋巴细胞核损伤的观察

OBSERVATION OF THE NUCLEAR DAMAGES IN LYMPHOCYTES OF HUMAN PERIPHERAL BLOOD INDUCED BY SOME ANTI-TUMOR DRUGS

本文用阿霉素、阿糖胞苷、氟尿嘧啶、氮芥及秋水仙素和长春新碱体外处理人外周血淋巴细胞,观察了这七种具诱变作用的抗癌药诱发的微核、核变形、核碎裂等核损伤变化。结取表明,七种抗癌药物均能引起多种核损伤指标的改变,并呈剂量依赖性增加,其中微核率、核异常率最明显°本实验结果并综合文献讨论后作者认为:应用核异常测试法评价化学诱变因子对人体的遗传毒性和潜在致癌性是可行的。

We studied the nuclear damages in lymphocytes of human blood induced in vitro by Adriamycin, Bleomycin-A5, Cytosine arabin-oside, 5-Fluorouracil, Colchicine, Vicristine and Mustinae hydrochloridum.The results show that the seven mutagenic antitumor drugs can cause obvious change of the nuclear damage in-dices (frequency of micronucleus, frequency of nuclear abnormalies, frequency of karyorrhetic nucleus, and frequency of irregular nucleus etc.) and dose-dependent increase are exhibited. Among the indices, the MNF and NAF are significant remarkably. Combining previous studies the experiment results suggest, the nuclear abnormaly test (NAT) should be available to evaluate the genetic toxicities and potential carcinogenicities to human exposed to chemical mutagenic factors.