PSS患者血管壁处组织型PA及其抑制因子的检测

Tissue-type PA and Its Inhibitor in Vessel Wall of Skin Lesions in Patients With PSS.

使用ＭｃＡｂ，以酶标抗体Ｂ－ＳＡ法检查５例ＰＳＳ患者典型皮损及５例健康对照正常皮肤的血管壁处ｔ－ＰＡ、ＰＡＩ－１、Ｆ＿Ⅷ和Ｆｂｇ。发现患者皮损血管壁处ｔ－ＰＡ阳性低于正常对照，而ＰＡＩ－１阳性则高于正常对照。提示ＰＳＳ患者皮损血管内外纤溶活性降低，血管内皮细胞及微血管结构损伤或功能障碍。

Tissue-type plasminogen activator (t-PA),plasminogen activator inhibitor-I(PAI-I),the eighth factor(F Ⅷ )and fibrinogen (Fbg) were localized by biotinstreptavidin (B-SA) method with the monoclonal antibodied(McAb)in the vessel wall of the skin lesions in 5cases of progressive systemic sclerosis(PSS)and the normal skin of 5 controls. The PSS patients had lower t-PApositive incidence and higher PAI-I positive incidencethan the controls. This suggested that there was decrease cutaneous and plasma fibrinolytic activity in theskin lesions of the patients with PSS and the injury or/and dysfunction of endothelial cells (EC)as well as microvasculature.