铁蛋白及其H、L亚基在肝癌及癌周分布的流式细胞定量分析

A QUANTITATIVE ASSESSMENT BY FLOW CYTOMETRY OF FERRITIN,FERRITIN-H AND L SUBUNIT IN HEPATOCELLULAR CARCINOMA AND ADJACENT NON-NEOPLASTIC LIVER

应用抗铁蛋白多抗、抗铁蛋白H、L亚基单抗和流式细胞测定技术,对23例手术切除的肝癌及癌周肝组织所含铁蛋白及H、L亚基进行定量免疫荧光研究,发现:1.癌周铁蛋白及H、L亚基与正常肝相差无显著性意义(p>0.05)。2.癌组织铁蛋白及其亚基均比癌周低(p<0.001)。3.癌、癌周的L亚基高于H亚基(p<0.001),L、H亚基有重要相关(癌:r=0.8,p<0.001;癌周:r=0.42,p<0.05)。4.有无肝硬化不影响肝癌及癌周铁蛋白及亚基含量。

We have used the poly-anti-ferritin antibody and monoclonal antibodies 2A4(spe-cific for the H subunit of ferritin) and LO3 (specific for the L subunit)and flow cyto-metry for quantitative immunofluorescent detection of ferritin in surgically resectedspecimens, consisting of hepatocellular carcinoma (HCC) and adjacent non-neoplasticliver from 23 patients with primary liver cancer. The results indicated that: (1)Therewas no significant difference(p>0.05) in ferritin, ferritin-H and L subunit betweennormal and adjacent non-neoplastic liver. (2) Ferritin, ferritin-H and L subunit waslower in HCC than in adjacent non-neoplastic liver (p<0.001). (3) In both HCC andnon-neoplastic liver, ferritin was more reactive to the anti-L than to anti-H antibody(p<0.00l) and there were significant correlations between H and L subunit in HCC(r=0.8, p<0.001) and non-involved liver (r=0.42, p<0.05) (4) There were no sig-nificant variations in ferritin, ferritin-H and L subunit between patients with andwithout cirrhosis (p>0.05).