恶性疟原虫FCC1/HN株185 kDa和82/41 kDa保护性抗原的超微结构定位

ULTRASTRUCTURAL LOCALIZATION OF 185 kDa AND 82/41 kDa PROTECTIVE ANTIGENS IN PLASMODIUM FALCIPARUM, FCC1/HN

用LR White树脂低温包埋感染人恶性疟原虫FCC1/HN株的红细胞,用保护性单克隆抗体F6-D3和F6-C2并结合蛋白A-胶体金探针免疫标记恶性疟原虫红内期185kDa和82/41kDa蛋白。结果表明单克隆抗体F6-D3识别的185kDa蛋白定位于游离的和细胞内的裂殖子表面以及未成熟裂殖体的细胞质、质膜及带虫泡膜。而单克隆抗体F6-C2识别的82/41 kDa蛋白则定位于未成熟裂殖体及成熟裂殖子的棒状体中。从超微结构上表明185 kDa和82/41 kDa保护性抗原分别为恶性疟原虫FCC1/HN株的裂殖子表面抗原和裂殖子棒状体抗原。

Plasmodium falciparum FCCl/HN-infected human erythrocytes were embedded with LR White resin at low temperature. The 185 kDa and 82/41 kDa proteins in erythrocytic stages of P. falciparum were then immuno-labeled by using the protective monoclonal antibodies (McAb)F6-D3 and F6-C2 with protein A-colloidal gold probe. The electron-microscopical observation showed that the 185kDa protein recognized by McAb F6-D3 was located on the surface of free and intracellular merozoites as well as the cytoplasm, plasma membrane, and parasitophorous vaccuole membrane of immature schizonts. The 82/41 kDa proteins identified by McAb F6-C2 was located within the rhoptries of immature schizonts and mature merozoites. These results demonstrated ultrastructurally that the 185 kDa and 82/41 kDa protective antigens were merozoite surface antigen and merozoite rhoptry antigens of P. falciparum FCC1/HN, respectively.