弓形虫感染鼠尿中循环抗原的检测

DETECTION OF CIRCULATING ANTIGEN IN URINE FROM MICE INFECTED WITH TOXOPLASMA TACHYZOITES

用快速ELISA检测弓形虫感染鼠的尿液,在轻、中、重三个感染组分别在感染后第5、4、3d检测到弓形虫循环抗原。实验结果显示弓形虫循环抗原的浓度与感染天数相平行,这在国内为首次报道。用免疫印迹法分析感染小鼠尿中弓形虫循环抗原,其特异区带为75、67、55、43、30、28、22 kDa。

Urine samples collected from three groups of mice infected experimentally with different numbers doses of Toxoplasma trophozoites were detected for the presence of Toxoplasma circulating antigen by using fast ELISA.The results showed that presence of circulating antigen in all three infected groups of mice in comparison with the normal control group. Toxoplasma circulating antigen was detected on days 5,4 and 3 after infection in light-,moderate- and heavy- infection groups,respectively. The concentration of circulating antigen was on a parallel with the duration of infection.Western blot analysis of the Toxoplasma circulating antigen in urine revealed the existence of seven specific bands with molecular weights of 75,67,55,43,30,28 and 22 kDa.