不同免疫调节剂对烫伤大鼠巨噬细胞功能的影响

Effect of different immunomodulators on macrophage function in rats with scald injury

目的探讨不同免疫调节剂对烫伤大鼠腹腔巨噬细胞(Mφ)功能的影响。方法应用 McAb APAAP 桥联酶标法、琼脂溶菌板法和 MTT 比色法等技术,分别测定了各组烫伤大鼠不同时相腹腔 Mφ各种功能的变化。结果①烫伤后大鼠腹腔 Mφ表面 Ia 抗原表达率降低,抗原提呈能力减弱,对念珠菌的噬菌率下降,溶菌酶活力减低,TNF 分泌增高,且伤后第10天比第5天更明显。以上结果与正常组比较,差异有非常显著意义(P<0.01)。②经不同免疫调节剂治疗后,各组烫伤大鼠腹腔 Mφ各种功能明显改善,各项指标与未用药的对照组比较,差异有非常显著意义(P<0.01)。结论烫伤后早期腹腔注射特异性免疫核糖核酸(iRNA)可明显改善伤鼠的免疫功能。

Objective To explore effect of different immunomodulators on macrophage(Mφ) functions in rats with scald injury.Methods The Mφ functions in various groups of rats with scald on the 5th and 10th day after scald were respectively determined with methods of McAb APAAP,agar bacteri- olytic plate and MTT colorimetry.Results The results showed that:①after scald,presenting rate of Ia antigen,ability of antigen presentation,phagocytic power to candida albicans and lysozyme ability of Mφ were significantly decreased,the capacity of Mφ secreting TNF was markedly increased compared with those of the normal group,and the differences were significant(P<0.01);②after the therapy with im- munodulators,the above functions of Mφ in rats with scald improved markedly.As compared with those of the control group,differences were significant(P<0.01).Conclusion The specific immune RNA,which is administered by intraperitoneal injection early after scald,can markedly improve immune functions of rats with scald.