缺氧对神经细胞缺氧诱导因子-1αmRNA表达的影响

The expression and clinical significance of HIF-1α mRNA in neurons under hypoxia

目的 揭示在缺氧状态下神经细胞缺氧诱导因子 - 1α(HIF - 1α)mRNA表达的时空规律。方法 对分离的新生SD大鼠大脑皮质神经细胞分别进行常规和缺氧培养 ,采用定量逆转录多聚酶链反应 (RT -PCR)检测神经细胞在不同缺氧时间HIF - 1αmRNA的表达。结果 神经细胞在常氧下HIF - 1αmRNA有极低的表达 ,在缺氧30min时表达量显著上升 ,6 0min达到高峰 ,90min后逐渐下降。与对照组比较 ,各缺氧组HIF - 1αmRNA的表达均有显著性差异 (P <0 .0 1)。结论 神经细胞在缺氧早期HIF - 1αmRNA表达有短暂。

Objective To explore the law of the expression of HIF-1α mRNA in neurons under hypoxia and its clinical implication. Methods The cortical neurons,prepared from neonatal SD rats, were cultured in normoxic and hypoxic condition respectively.Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was employed to examine the expression of HIF-1α mRNA in neurons. Results In normoxic condition the expression of HIF-1α mRNA in neurons was at a very low level.While under hypoxia, the level of expression increased rapidly and transiently, with the maximal expression at 90 min after hypoxia. Compared with the normoxic group, the expression of HIF-1α mRNA in the hypoxic groups all had a significant difference (P<0.01). Conclusion These results demonstrate the expression of HIF-1α increases rapidly and transiently under hypoxia.