苦参碱对K562细胞端粒酶hTERT-mRNA表达及其酶活性影响作用的研究

Effects of Matrine on hTERT- mRNA Expression and Telomerase Activity in K562 Cells

目的：观察苦参碱对 K562细胞 hTERT- mRNA表达及端粒酶活性的影响作用 ,并明确两者的相关性。方法：以 0.1～ 2 mg/ml苦参碱处理 K562细胞 48h后， RT- PCR检测其 hTERT- mRNA表达，同时行 TRAP- PCR- ELISA端粒酶活性检测。结果 :K562细胞为一强端粒酶活性细胞株，在浓度分别为 0.1、 0 5、 1 0、 2 0 mg/ml苦参碱作用后， K562细胞 hTERT- mRNA表达明显受抑，同时伴有端粒酶活性下降，抑制率分别为 0 3％、 13 0％、 91 7％和 98 6％。结论：苦参碱可降低 K562细胞 hTERT- mRNA表达 ,同时伴端粒酶活性下降；端粒酶活性强度与 hTERT- mRNA表达有关。

Objective:This study was designed to investigate the effects of matrine on hTERT- mRNA expression and telomerase activity in K562 cells and their relationship. Methods: The hTERT- mRNA expression was determined by RT- PCR assay in untreated and matrine- treated K562 cells, and telomerase activity was analyzed by TRAP- PCR- ELISA assay. Results: The hTERT- mRNA expression of K562 cells was significantly inhibited when treatment with matrine, while telomerase activity was decreased. Conclusion: Matrine can inhibit the hTERT- mRNA expression and telomerase activity of K562 cells. Telomerase activity might be related to the expression of hTERT- mRNA.