应用聚合酶链反应-反相膜杂交技术检测结核分枝杆菌

Detection of mycobacterium tuberculosis with PCR－Reversed phase membrane hybridzation RPMH technigue Detection of mycobacterium tuberculosis with

目的 探讨聚合酶链反应（PCR）－反相膜杂交技术检测结核分枝杆菌的价值。 方法 收集52例活动性肺结核病人和12例非结核病人的痰标本，以双盲法进行痰涂片抗酸染色检查、结核菌培养、PCR－反相膜杂交技术，PCR－琼脂糖凝胶电泳技术检查对比。 结果 涂片法、培养法，PCR－反相膜杂交法的敏感性分别为28．8％、42．3％、82．7％，PCR－反相膜杂交法的敏感性显著高于培养法和涂片法（P＜0．01）。PCR－反相膜杂交法和PCR琼脂糖凝胶电泳法的敏感性分别为82．7％和84．6％，特异性分别为91．7％和83．3％，前者特异性与后者相比差异有显著性（P＜0．01）。 结论 PCR－反相膜杂交技术可应用于临床检测结核分枝杆菌。

 Objective To explore the value of PCR－reversed phase membrane hybridization PCR－RPMH technique for detecting Mycobacterium tuberculosisM．TB． Method The sputum samples of 52 patients with active tuberculosis and 12 without tuberculosis were detected by double－blindly with thick smear acid－fast staining Culture PCR－RPMH and PCR－agar gel electrophoresis PCR－AGE technique． Result The sensitivity of smear、 culture、 PCR－RPMH was 28．8％、42．3％、82．7％ respectively． PCR－RPMH technique was obviously higher than that of other two methodsP＜0．01． The sensitivity of PCR－AGE was 84．6％ by． The specificity of PCR－RPMH and PCR－AGE were 91．7％ and 83．3％ respectively between of them showed significantly different P＜0．01． Conclusion PCR－RPMH technique is valuable for the detection of M．TB and clinical application． 