吡拉西坦长期大剂量用药对小鼠学习记忆的影响

Effect of long term and high-dose piracetam administration on learning and memory in mice

目的 :了解吡拉西坦长期大剂量用药的促进记忆作用。方法 :吡拉西坦 6 0 0mg/kg连续用药 30天 ,分别用丫迷宫法测定小鼠的空间辨别能力和用高效液相荧光法 (HPLC)测定脑内谷氨酸 (Glu)及γ 氨基丁酸 (GABA)水平。结果 :吡拉西坦长期用药仍能明显提高小鼠空间辨别能力 ,其作用可被氯胺酮所拮抗 ,脑内Glu和GABA水平无明显改变。结论 :吡拉西坦长期用药时仍有明显促进记忆作用 ,而脑内Glu和GABA递质水平无异常改变 。

Objective:To inquire into effects of long term and high dose piracetam administration on learning and memory in mice .Methods:The mice were given piracetam 600mg/kg for 30days ig and then acquisition of spatial discrimination of mice was tested by Y maze method. The glutamate and GABA content in mice brain was measured by high performance liquid chromatography with fluorcscence detector.Results:Long term and high- dose of piracetam administration can significantly promote the acquisition of spatial discrimination mice. ketamine ( 8. 5mg/ kg ip) ,a selective NMDA receptor antagonist inhibited the anti amnesic activity of piracetam. There was no influence on the Glu and GABA content in mice brain.Conclusion:Piracetam administration for a long time still brought about anti amnesic effect with no influence of Glu and GABA content in mice brain.The facilitatory effect of piracetam on memory may be mediated by glutamate receptor.