烫伤早期大鼠垂体前叶P物质神经的分布变化规律

Changes of substance P-like immunoreactive nerve fibers in the anterior pituitaries of rats after scald

目的 :观察烫伤早期大鼠垂体前叶P物质神经的变化规律 ,为探讨烧伤后垂体水平调节全身反应的作用机制提供实验资料。方法 :采用大鼠 30 %TBSAⅢ度烫伤模型 ,于伤后 1,2 ,6 ,10 ,12 ,2 4,72h 7个不同时间点 ,应用免疫组织化学技术对垂体前叶SP肽能神经染色后观察其形态特征改变 ;利用图像分析测定比较SP神经分布数量及覆盖面积的变化。结果 :SP肽能神经纤维的形态于伤后镜下观察可见明显变化 ;伤后 1h表现为神经数目增加 ,伴有神经纤维的膨体密度、分枝等明显增多 ;2h后大幅度减少 ,2 4h后趋于恢复。神经分布数量及覆盖面积的基本变化趋势是伤后 1h升高 ,随后降低 ,伤后 12h降至最低点 ,然后呈现回升 ;相关分析显示神经数量与覆盖面积间的动态变化呈正相关。结论 :烫伤早期大鼠垂体前叶的SP神经参与了全身神经内分泌的功能调节过程。

Objective:To observe the changes of substance P-like immunoreactive(SP-LI)nerve fibers in the anterior pituitary of rats after scald.Methods:The SP-LI nerve fibers of anterior pituitary were displayed by immunohistochemistry at different observation time points in 72 hours after the rats were scalded to 30% total body surface area(TBSA) of full thickness burn.The numbers and cover areas of SP-LI nerve fibers were measured respectively in anterior pituitaries by image analysis.[WT5HZ]Results:SP-LI nerve fibers with more varicosities and branches increased by 1 hour postscald;then decreased obviously by 2 hours postscald and remained at the low distribution till 24 hours postscald.Correspondingly,the cover areas of nerve fibers became larger by 1hour postscald,and less with the time going,which showed the lowest by 12 hours postscald.Conclusion:SP-IL nerve fibers of anterior pituitary might be involved in the response of rats to scald stress.