用微小卫星引物PCR鉴定红色毛癣菌和须癣毛癣菌

PCR fingerprint identification of Trichophyton rubrum and Trichophyton mentagrophytes using single primers specific to minisatellites and simple repetitive DNA sequence

目的 :观察红色毛癣菌和须癣毛癣菌临床分离株 DNA PCR指纹的差异 ,找出一种区分红色毛癣菌和须癣毛癣菌的基因分类方法。 方法 :采用寡核苷酸重复序列 (GACA ) 4、(GTG) 5 及 M13中心序列 (5′- GAGGGTGGCGGTTCT- 3′) 3种引物 ,对 2 3株红色毛癣菌和 11株须癣毛癣菌临床分离株的 DNA进行 PCR扩增 ,观察产物电泳带型的差异。结果 :在 3种引物的扩增产物中 ,均可见红色毛癣菌和须癣毛癣菌呈现出不同的 DNA指纹 ,其中 ,以引物 (GACA) 4扩增的条带差异最为清晰。结论 :红色毛癣菌和须癣毛癣菌可以用 PCR方法加以鉴别 ,以 (GACA ) 4作引物区分这两种菌较为合适。

Objective: To observe the difference between the species Trichophyton rubrum and Trichophyton mentagrophytes. Methods: Three primers, including (GACA) 4, (GTG) 5 and M13 core sequence (5′ GAGGGTGGCGGTTCT 3′), were used to distinguish variations among 23 clinical isolates of T. rubrum and 11 of T. mentagrophytes. Results: Different PCR fingerprinting were seen between T. rubrum and T. mentagrophytes by using 3 different primers, especially amplification with primer (GACA) 4 could give more distinct bands. Conclusion: T. rubrum and T. mentagrophytes can be distinguished by PCR, (GACA) 4 is the most suitable primer. [