铁路危险品货运站空气污染物对肺细胞DNA的损伤作用

Study on the DNA Damages in Type Ⅱ Lung Cells by Air Pollutants in a Railway Transport Station of Chemical Dangerous Goods

[目的 ]探讨铁路危险品货运站空气污染物对大鼠肺Ⅱ型细胞DNA的损伤作用。 [方法 ]应用大鼠肺Ⅱ型细胞的原代培养技术 ,结合四氮甲基唑蓝 (MTT)比色法、DNA交联试验和单细胞凝胶电泳试验 ,检测了铁路危险品货运站空气污染颗粒提取物对大鼠肺Ⅱ型细胞生长的抑制作用及对DNA的损伤情况。[结果 ]在 1 0 2、2 0 3、4 0 6、8 13mg/ml的浓度范围内 ,颗粒提取物能引起大鼠肺Ⅱ型细胞DNA产生双链间的交联和单链断裂 ,并存在剂量 -反应关系 (r交联 =0 981,r断裂 =0 987,P <0 0 1)。 [结论 ]铁路危险品货运站空气污染颗粒提取物对大鼠肺Ⅱ型细胞DNA具有明显的损伤作用 ,其损伤程度与染毒浓度和染毒时间有关。

Objective] The aim of this study was to explore the DNA damages induced by air pollutants in a railway transport station of chemical dangerous goods (RTSCDG) in rat type Ⅱ lung cells. [Methods] MTT assay and the ethidium bromide fluorescence assay and the single cell gel electrophoresis assay were applied respectively to detect the cytotoxicity, DNA cross links and DNA single strand breaks in rat type Ⅱ lung cells after exposure to the particle extracts of air pollutants in RTSCDG. [Results] The rat type Ⅱ lung cells were treated with extract of air pollutants of RTSCDG at a single concentration of 1 02,2 03,4 06,8 13mg/ml,increase in DNA cross links and DNA single strand breaks and the dose response relationship were found after treatment ( r cross links =0 981, r breaks =0 987, P <0 01). [Conclusion] The air pollutants in RTSCDG may result in DNA damages in rat type Ⅱ lung cells.