p53和mdm2在骨巨细胞瘤中的表达

EXPRESSION OF p53 AND MDM2 PROTEIN IN HUMAN GIANT CELL TUMORS OF BONE

目的 :探讨 p5 3和 mdm 2在骨巨细胞瘤 (GCT)的表达及与 GCT病理分级和复发的关系。方法 :应用 SP免疫组织化学方法检测 p5 3和 m dm 2在 5 2例 GCT(GCT按 Jaffe分级 : 级 15例、 级 2 5例、 级 12例 )中的表达。结果 :14例 p5 3表达阳性 ,阳性率 2 6 .9% ,18例 m dm 2表达呈阳性 ,阳性率 34.6 %。其阳性表达与病理分级均无显著性差异 (P值分别为 0 .6 99;0 .871)。 p5 3和 mdm 2在复发和无复发的病例中 ,阳性表达率分别为 46 .2 %、2 0 .5 %和 6 1.5 %、2 5 .6 %。两者同时阳性表达的有 7例 ,p5 3和 m dm 2同时过表达与 GCT复发有高度显著性差异 (P=0 .0 0 8)。结论 :p5 3和 m dm 2在 GCT中的表达与病理分级无关而与其复发有关。

Objective:To investigate the relationship of p53 and mdm2 protein overexpression with pathological types and recurrence.Methods:Monoclonal antibodies directed against p53 and mdm2 proteins were used to measure expression of these proteins in the muclei of cells from sections of the giant cell tumor of bone.Rusults:14 of 52 tumors overexpressed p53 protein and 18 of 52 tumors had abnormally high levels of mdm2 protein.7 cases had abnormally high levels of both p53 and mdm2 proteins based upon immunoreactivity with these antibodies.There was a striking statistically significant correlation between the overexpression of p53 and mdm2 proteins in the same tumor and recurrence(P=0.008).However,there was no association between p53 and mdm2-positive cases and pathological types.Conclusion:There was a significant correlation between of p53 and mdm2 protein in the giant cell tumor of bone and pathological types.