胶质源性神经营养因子对大鼠脊髓不完全损伤后运动功能的保护作用

The protective effect of GDNF on spinal motor function after incomplete spinal cord injury in rats

目的 :探讨胶质源性神经营养因子 (GDNF)对大鼠脊髓损伤后运动功能恢复的影响。方法 :动物分为对照组、假手术组、生理盐水 (NS)组及GDNF组 ,改良Allen方法致脊髓不完全损伤 ,蛛网膜下腔分别给予NS及GDNF2 0 μl,伤后 1d ,3d ,7d ,10d ,14d及 2 1d评定下肢运动功能 (Tarlov评分及Rivlin斜板 )。 结果 :假手术组与对照组、假手术组自身无明显变化 (P >0 .0 5 ) ,NS组及GDNF组伤后运动功能有明显障碍 ,NS组 14d、GDNF组 7d以后脊髓功能明显改善 ,10d以后GDNF组运动功能改善显著超过NS组 (P <0 .0 1)。结论 :GDNF能够挽救损伤脊髓运动神经元 。

Objective:To investigate the effect of glial cell line-derived neurotrophic factor (GDNF) on spinal motor function after incomplete spinal cord injury(SCI) in rats.Method:The rats were divided into four groups of contrast, false operation, NS and GDNF. NS and GDNF of 20μl respectively were supplied through subarachnoid cavity after T13 segments of spinal cord were injured by modified Allen crush method. Motor function of lower limbs was evaluated using Tarlov'grading and Rivlin'oblique plate at 1d,3d,7d,10d,14d and 21d after SCI.Result:There was no obvious difference between false operation group and contrast group, and no difference among false operation groups at every assessing time( P >0.05). Distinct handicap of motor function in lower limbs was watched both in NS group and GDNF group as soon as SCI. As time going on, motor function came back gradually. Marked improvement of motor function comparing that of 1 day postoperatively was seen 7 days in GDNF group and 14 days in NS group after SCI. The amelioration in GDNF group exceeded notably that in NS group 10 days after SCI ( P <0.01). Conclusion:GDNF could survive injuried spinal motorneuron, promote restoration of spinal motor function after incomplete SCI in rats.