急性白血病P^(15)基因异常及甲基化研究

Homozygous deletion and methylation of P^(15)gene in acute laukemia

用聚合酶链反应 (PCR)方法扩增 P1 5基因外显子 1,再用限制性内切酶 - PCR方法检测 P1 5基因甲基化。结果 48例患者中 P1 5基因失活者共 2 7例 (5 8.92 % ) ,AL L2 1例中有 12例 (5 7.41% ) ,ANL L2 7例中 15例(5 5 .5 6 % )。 P1 5 基因以甲基化失活为主。 P1 5 Cp G岛甲基化在各型急性白血病中均有很高的发生率 ,可以作为各型急性白血病通用的微量残留病 (MRD)指标 ;白血病缓解期 P1 5 Cp G岛甲基化仍为阳性可能预示着复发。认为 P1 5基因失活的检测对于探讨急性白血病的发病机制。

To investigate the role of P 15 gene in the pathegenesis of leukemia,48 patients with newly diagnosed acute leukemia were studied PCR technique was used to detect homozygous of P 15 gene,restriction enzyme PCR technique used to detect gene methylation This results showed P 15 gene inactivation was detected in 28 of the 48 patients For all patients,methylation was the major pathway of P 15 gene inactivation Patients with P 15 gene inactivation [KG(-25]had a poor response to chemotherapy and a lower remission This suggests that the methylation of P 15 gene islands occurs very commonly in every types of AL It can be used as a gene marker for AL and for minimal residual disease in CR The inactivation of P 15 gene plays a key role in the pathogenesis of acute leukemia