蔬菜灰霉病菌对腐霉利抗药性变异及其治理

STUDY ON RESISTANCE OF BOTRYTIS CINEREA TO PROCYMIDONE

蔬菜灰霉病菌(Botrytis cinerea)分生孢子经紫外光灯照射,可获得10^(-4)～10^(-5)频率的腐霉利抗药性突变体。获得的13个抗性突变体的抗性水平平均EC_(50)值为18.45μg/ml,EC_(90)值为305.17μg/ml。抗性突变体的可溶性蛋白谱带比其亲本敏感菌株多2～4条,其中Rf=0.38的蛋白谱带是抗性菌株所拥有而敏感菌株所共同缺乏的。抗性菌株对腐霉利抗药性稳定遗传,具有与亲本菌株相同的致病性。苗期抗、感测定结果表明,灰霉克杀菌剂对腐霉利突变体具有明显的克抗作用。田间抗性菌治理使用灰霉克400～800倍对大棚番茄灰霉病平均防效为95.93％～75.8％,优于腐霉利600倍的平均防效(61.32％)。

After mutagenizing by ultraviolet light Botrytis cinerea produced 10-4 - 10-5 resistant mutants of procymidone. The mean resistant level of 13 mutant strains was 18.45*g/ml(EC50) . The soluble proteinogram bands of resistant mutants were 2-4 more than that of their parent sensitive strains. Among which, the resistant strains owned the soluble proteinogram band of Rf = 0.38, but it was not showed in sensitive strains. The resistance of strains was stable hereditary. They have same reproductive capacity and pathogenicity as parent strains. The average control result of Huimeike were 95.93% - 75.8% and were better than the results of procymidone.