He-Ne激光影响纯钛种植体骨性愈合的实验研究

Experimental Study Relationship Between He-Ne Irradiation and Bony Union of Pure Titanium Implant Planting in Bone

目的 :探讨He-Ne激光对种植体骨性愈合影响。方法 :将24只白兔手术暴露双侧胫骨内侧 ,每侧胫骨内植入直径2mm ,高5mm纯钛种植体4棵。术后随机选一侧每天用15J/cm2He -Ne激光照射术野15分钟为实验组 ,另一侧为对照组。于照射后第1、2、3、4周各处死6只动物。行种植体界面光镜、电镜观察及成骨量定量分析。结果 :光镜及电镜观察显示实验组中骨胶原纤维、骨小梁、成骨细胞、破骨细胞、钙盐沉积均比对照组较早出现 ,数量也明显增多。定量组织学分析除术后2周外 ,成骨量实验组均比对照组显著性增加。结论

Objective:To study the He_Ne laser irradiation could be possible to promote the bone formation in the bone_implant interface.Methods:24 white rabbits were operated on the internal surface of the tibias.Four titanium implants were inserted in tibia bone at each side . Tow sides of each rabbit were divided as experiment side and control side.Every implant was irradiated with 15J/cm2 He_Ne laser for 15 minutes every day in experiment side and no irradiated in control side.6 animals samples were adopted after 1,2,3 and 4 weeks after irradiation.The bone_implant interfaces were observed under the light microscope(LM) and the scanning election microscope(SEM).The result of quantitative analysis of bone formation content in the bone_implant interface was achieved.Results:The LM and the SEM study showed that bone fiblers,bony trabecula,skeletogenous cell and calcium salt aggrandizement appeared earlier in experimental group than in control group.The formation of new bone at bone_implant interface in experimental group was aggrandized remarkably than in control group irradiated tow weeks later. Conclusion:He-Ne lasers irradiation was a reliable and effective method for accelerating bone formation in bone_implant interface.