摘要
目的采用丝状噬菌体表达载体,构建表达于丝状噬菌体尾丝蛋白P3N末端的随机十肽库.方法人工合成编码随机十肽的寡核苷酸片段及两条分别有SfiI和NotI酶切位点的引物,通过PCR反应得到其双链片段,将其克隆入载体pHEN1中,将重组产物转化入大肠杆菌TG1,构建成随机肽库.结果所建肽库含1×109个集落形成单位(cfu).随机挑取8个克隆测序,其核苷酸序列及推断出的氨基酸序列均随机.结论成功构建了有较高库容量的噬菌体随机十肽库.
To construct a random decapeptide library by phage display method using filamentous phage vector. Methods Nucleotide fragments coding random decapeptide and two primers were synthesized. Double strained DNA fragments were produced by polymerase chain reaction. The recombinant pHEN1 containing random oligonu-cleotides were transformed into TG1. Results The total clone of the constructed decapeptide library was 1×109. Sequencing of 8 randomly selected clones from library revealed randomized distribution of their uncleotides and deduced amino acids. Conclusion The random phage display decapeptide library with high containing volume has been successfully constructed.
出处
《传染病信息》
2001年第1期24-25,共2页
Infectious Disease Information
关键词
噬菌体表面展示
肽库
随机十肽
Phage display Peptide library Random decapeptide
