应用高效薄层色谱法检测母乳中神经节苷脂

Detection of gangliosides in human milk with a high performance thin layer chromatography (HPTLC)

目的 检测泌乳期母乳中神经节苷脂成分 ,并评价其生物学意义。方法 应用高效薄层色谱法对 6 8份母乳标本进行了检测。结果 母乳中总神经节苷脂结合唾液酸含量为 7 8～ 10 4μg/ml。在母乳中检测出 4种神经节苷脂 ,分别为GM3、GD3、GX1及GX2。GX1及GX2为新发现的神经节苷脂 ,对GA1抗体呈阴性反应 ,推测其可能为C 通路神经节苷脂。GM3与GD3为主要神经节苷脂 (占总量 5 0 %～ 6 5 % )。GM3在分娩后第 8天时明显增高 ,GD3则明显降低。随分娩后天数增加GM3与GD3变化有显著性。结论 母乳中存在GM3、GD3、GX1及GX2 4种神经节苷脂 ;

Objectives To determine the ganglioside composition in human milk during lactation,and to evaluate the biological significance. Methods The gangliosides extracted from 68 milk samples were examined with a high performance thin layer chromatography (HPTLC). Results The concentration of total lipid bound sialic acid was 7 8 10 4 μl/ml Four gangliosides were found and designated as GM3,GD3,GX1 and GX2,respectively GX1 and GX2,which had not been described previously,were tentatively assumed to be gangliosides of the c pathway because they did not react to the GA1 antibody after sialidase treatment GD3 and GM3 were the major gangliosides in human milk (accounted for 50%～65%) CM3 decreased sharply at eight days postpartum Both GM3 and GD3 changed significantly during lactation (GM3, r =0 65, P <0 01; GD3, r =-0 58, P <0 01). Conclusions Four gangliosides,GM3,GD3,GX1 and GX2 were found in human milk Both GM3 and GD3 changed significantly during lactation