大鼠脊髓前角内NMDA受体的定位与生后发育

LOCALIZATION OF NMDA RECEPTORS IN THE VENTRAL HORN =OF SPINAL CORD OF ADULT RAT AND DURING POSTNATAL DEVELOPMENT

目的 探讨 NMDA受体亚基 (NMDAR1和 NMDAR2 A / B)在大鼠脊髓前角的细胞学定位和生后发育特征。 方法 免疫组织化学染色技术。 结果 NMDAR1与 NMDAR2 A / B免疫反应产物丰富地分布在脊髓前角 Rexed 和 层内 ,主要定位于神经元胞体、树突和轴突样纤维终末上。生后早期的 NMDAR1和 NM-DAR2 A/ B表达具有明显的动态变化 ,生后 7d(P7)的表达微弱 ,随后逐渐上调 ,至 P2 1达高峰水平。然后维持此水平至成年。 结论 NMDAR1和 NMDAR2 A是构成脊髓前角神经元功能性 NMDA受体的重要亚基 ,NMDA受体可能参与生后早期运动神经元成熟或可塑性调控过程。

Objective To investigate the cellular localization and developmental patterns of NMDA receptor subunits(NMDAR1 and NMDAR2A/B) in the ventral horn of rat spinal cord during postnatal stages. Method Immunohistochemical staining method was used in the present study. Results NMDAR1-or NMDAR2A/B-immunorectivity is abundantly observed in the lamina Ⅷ and Ⅸ of ventral horn.The immunopositive product is localized mainly in the neuronal somata,dendrites and axon terminals.Dynamic changes of NMDA receptor expression occur in early postnatal stages.NMDA receptor subunits express weakly at postnatal 7 days (P7),goes up at P14,reaches maximum level at P21,then remains this level until adult. Conclusion Neuronal functional NMDA receptors are composed of NMDAR1 and NMDAR2A in heteromeric configuration in the ventral horn of spinal cord;NMDA receptors may be involved in motor neuron maturation and plasticity during postnatal development.;