摘要
目的 观察大鼠三叉神经中脑核 (Vme)神经元内磷酸激活的谷氨酰胺酶 (PAG)和小白蛋白 (PV)的共存关系 ,以及前强啡肽原 (PPD)样和前脑啡肽原 (PPE)样阳性终末与 PAG和 PV共存神经元之间的联系。 方法 免疫荧光组织化学双重和三重染色技术 ,在激光共聚焦显微镜下观察。 结果 在 Vm e内可见大量 PAG样和 PV样免疫反应神经元 ,吻尾方向上几乎在其全长出现 ,多为大的假单极神经元。许多 PAG样阳性神经元同时呈 PV样免疫反应 ,双标细胞占全部标记细胞的 95 %以上。在激光共聚焦显微镜下观察到 ,少量 PPD样或 PPE样阳性终末围绕在 PAG样和 PV样双重标记的 Vme神经元胞体周围 ,并与之形成密切接触。 结论 在面口部本体感觉信息由 Vme向更高一级神经元传递的过程中 ,PV可能和谷氨酸一起发挥着重要的作用 ,与此同时 Vm e神经元还可能接受中枢内强啡肽样和脑啡肽样神经终末对它的调控。
Objective To observe the co-localization of phosphate activated glutaminase(PAG) and parvalbumin(PV) in neurons,and the connections between preprodynorphin-like immunoreactive(PPD-LI) or preproenkephalin(PPE)-LI terminals and coexistent neurons of PAG-LI and PV-LI in the mesencephalic trigeminal nucleus(Vme) of the rat. Methods In the present study,the immunofluorescence histochemical double-staining and triple-staining technique were used.The staining results were observed under a confocal laser scanning microscope. Results Many neuronal cell bodies through the whole rostrocaudal extent of Vme showed PAG-LI and PV-LI.The majority of them were large pseudounipolar neurons.A considerable number of PV-LI neurons expressed PAG-LI,cell counts showed that more than 95% of PV-I neurons were immunostained with PAG-LI.Confocal laser scanning microscope further revealed that axonal varicosities with PPD-LI or PPE-LI were found to make close contact with neuronal cell bodies of Vme showed both PAG-LI and PV-LI. Conclusion The present results indicated that the glutamate and PV are involved in the transmission of the trigeminal proprioceptive sensory singals from the Vme to the higher centers,simultaneously,dynorphin-and enkephalin-immuoreactive terminals may also exert modulatory influence on the primary afferent cell bodies of the Vme.;
出处
《解剖学报》
CAS
CSCD
北大核心
2001年第2期109-113,T002,共6页
Acta Anatomica Sinica
基金
国家自然科学基金!资助项目 (39870 2 6 2 )