纯视细胞移植术后RCS鼠视皮质内γ-氨基丁酸的分布

OBSERVATION OF THE DISTRIBUTION OF γ-AMINOBUTYRIC ACID INTHE VISUAL CORTEX OF THE RECONSTRUCTED RCS RAT′S RETINABY TRANSPLANTING PURE PHOTORECEPTOR CELLS

目的 通过成功纯视细胞移植 ,观察 RCS鼠视皮质内抑制性神经递质 γ-氨基丁酸 (GABA)的分布和变化。 方法 取同龄 Wistar/皇家外科学院 (Royal College of Surgeon,RCS)鼠为供 /受体 ,准分子激光切削法制备视细胞层 ,外路途径移入 RCS鼠的视网膜下腔 ,术后 2周取 RCS鼠手术组眼、手术对照组眼、RCS对照组眼及其视皮质分别做冰冻切片 ,免疫组织化学染色法染色 ,普通光学显微镜下观察。 结果 视细胞移植术后 2周 ,移植视细胞存活 ,外丛状层重建 ,与手术对照眼、RCS对照眼相比 ,重建视网膜受体 RCS鼠对侧视皮质内 GABA免疫反应神经元数量较多 ,而同侧视皮质内无 GABA免疫反应神经元。 结论 成功纯视细胞移植可以重建 RCS鼠的视觉传导通路 ,移植的纯视细胞使 RCS鼠视皮质内 GABA免疫反应的分布发生变化。

Objective We observe the γ-aminobutyric acid(GABA)′s variation in the visual cortex of Royal College of Surgeons Rat(RCS rat)′s retina following the photoreceptor cell transplantation. Method The healthy Wistar rats′photoreceptor cells were grafted into the sub-retinal space of the left eye of the RCS rat by outer-path way.Two weeks later,host animals were sacrificed.Both sides of visual cortex were processed for light microscopic analysis.So did the 74 days Wistar rats and 74 days RCS rats.Then immunocytochemistry was used to determine the localization of the amino acid neurotransmitters(γ-aminobutyrate,GABA).Results After two weeks of transplantation,the transplanted photoreceptor cells survived.On the opposite side of the visula cortex in the grafted RCS rats,GABA-like immunoreactivity was intensely stained.But the same side of the visual cortex in the grafted RCS rats,GABA-like immunoreactivity could not be observed.Conclusion These results suggest that we can rebuild the visual pathway by transplanting the photoreceptor cells.In the visual cortex of the grafted RCS rats, GABA- like immunoreactivity may indicate that the visual cortex had reflection to the stimulation after photoreceptor cells had been grafted.