328例血清TT病毒DNA及其IgG抗体的检测

Detection of TTV DNA and TTV IgG in 328 serum samples

目的 了解不同人群中TT病毒 (TTV)感染情况。方法 根据Okamoto报道的TTV全序列设计引物 ,建立TTVDNA套式聚合酶链反应 ,利用该法对 81例正常人、92例献血员、12 3例甲～庚型肝炎 ,32例非甲～庚型肝炎病人进行TTVDNA检测 ,同时用ELISA法检测抗TTVIgG。结果 TTV在以上四种人群中阳性率分别为 2 5 %、2 2 %、19 5 %、2 8 1% ,抗TTVIgG的阳性率分别为 1 2 %、3 7%、2 6 8%、34 4% ,前者与后两者比较差异存在显著性 (P <0 0 5 ) ;重叠感染中TTV合并HBV的二重感染率最高为 75 0 %。结论 不同人群均存在TTV感染 ;正常人群和职业献血员存在健康携带状态 ;甲～庚型肝炎和非甲～庚肝炎病人为高危人群 ;TTV可与各型肝炎存在重叠感染 ;TTV除经血传播外 ,存在其他传播途径 。

Objective To investigate the prevalence of TT virus(TTV) in a variety of populations in China.Methods TTV DNA and anti TTV IgG were simultaneously tested by PCR and ELISA.Results The positive rate of TTV DNA in the samples of 81 normal subjects ,92 blood donors ,123 hepatitis A-G patients and 32 hepatitis non A-G patients were 2\^5%,2\^2%,19\^5% and 28\^1%,respectively; and anti\|TTV IgG was 1\^2%,3\^7%,26 8% and 34\^4%,respectively.There were significant differences between the normal and the blood donor group and the hepatitis group;and among multi\|infectious cases,the infection rate of TTV with HBV was as high as 75\^0%.Conclusion The investigation indicated that TTV infection was very popular in different groups.Some of the normal and blood donor groups infected with TTV could become healthy carries of virus.And the hepatitis patients were the high risk group.In addition,TTV may be transmitted via other pathways besides blood.