减毒麻疹病毒联合拉米夫定体内抗鸭乙型肝炎病毒作用的研究

Anti-virus effect of combination of attenuated measles virus with 3TC on duck hepatitis B model

目的 观察减毒麻疹病毒与拉米夫定 (3TC)联合用药在鸭体内抗乙型肝炎病毒的作用。方法 以感染鸭乙型肝炎病毒 (DHBV)的广州麻鸭为实验动物模型 ,在感染后分别给予口服3TC、肌注麻疹疫苗和联合使用麻疹疫苗与 3TC ,并以无环鸟苷 (ACV)口服作为用药对照组 ,肌注生理盐水作为病毒对照组。于给药前 (T0 ) ,给药第 5天 (T5) ,第 10天 (T10 )及停药第 3天 (T13)留取血清 ,采用斑点杂交方法测定DHBVDNA。结果 3TC组在T5和T13 两点病毒量略有下降 ,但与T0 比较 ,差异均无显著性 (P >0 0 5 ) ;麻疹疫苗组在给药早期病毒量无下降 ,T13 点时病毒量与T0 比较有显著下降 (P <0 0 1) ,与病毒对照组的同期比较 ,差异也有显著性 (P <0 0 5 ) ;麻疹疫苗与 3TC联合使用组在给药早期 (T5)出现病毒量明显下降 (与T0 比较 ,P <0 0 1) ,其A值与有确定疗效的ACV组T5比较也无差异 (P >0 0 5 ) ,停药第 3天时的病毒量与给药前及病毒对照组同期比较 ,差异均有显著性 (P <0 0 1,P <0 0 5 )。结论 减毒麻疹病毒与 3TC联合给药较单独给药对DHBV

Objective To evaluate the anti virus effect of combination of attenuated measles virus with 3TC on duck hepatitis B model. Methods Guangzhou brown spot ducklings infected with DHBV were divided into three groups. Drug treatments including measles vaccine,3TC and both measles vaccine and 3TC were given in the 13th day after the infection,respectively. Serum samples were obtained from all ducklings before,during and after treatment at different times and stored at -70 ℃. DHBV DNA was detected by Dot blot hybridization.Results Compared with the levels of DHBV DNA before and after treatment,no statistic difference was found in group of 3TC alone. The levels of DHBV DNA were not changed in group of measles vaccine alone in early stage, but decrease of DHBV DNA occurred after stopping treatment (comparison of the levels T 13 with before treatment and T 13 of positive group, P <0.01 and P <0 05,respectively). DHBV DNA level in group of measles vaccine combined with 3TC was obviously lower at 5th day after the treatment than in both measles vaccine and 3TC alone groups at the same time( P<0 05 and P <0.01,respectively),and it remained low when the treatment was discontinued. Conclusion Attenuated measles virus combined with 3TC may have early and long lasting inhibiting effect on the replication of DHBV.