中国部分城市A组轮状病毒感染状况调查及VP7血清型分析

Investigation of group A rotavirus infection in several cities in China and prevalence of VP7 serotype

目的 研究我国A组轮状病毒感染状况及VP7血清型分布。方法 利用PAGE电泳检测北京、沈阳、新乡、上海、深圳、广州六个城市 1997和 (或 ) 1998年秋冬季婴幼儿腹泻A组轮状病毒的感染状况 ,并采用RT PCR方法对 4种主要的VP7血清型 (G1、G2、G3和G4型 )进行分型。结果 374份标本中有 181份为轮状病毒阳性 ,检出率 48 4%。各地标本的检出率在 32 %～ 6 3 %之间 ,北京、上海地区两年的检出率基本近似。阳性标本经PCR分型方法鉴定G1、G2、G3和G4型的感染率分别为 89 5 %、6 1%、2 8%和 0。有两例为G1和G2型混合感染 ,另有 1例经测序证实为G9型。各地之间、每年之间RV的流行状况略有差异。结论 G1。

Objective To sutdy the prevalence of Group A rotavirus infection in China.Methods 374 samples from infants with diarrhea were collected from six cities (Beijing,Shenyang,Xinxiang,Shanghai,Shenzhen and Guangzhou)in autumn and winters of 1997 and/or 1998.The incidence of rotavirus infection and the prevalence of the four dominant serotypes G1,G2,G3,G4(VP7 serotype)were detected by PAGE and RT\|PCR.Results Of the 374 samples detected,181 were rotavirus positive.The positive rate was 48\^4%,ranging from 32%～63% in different cities.The positive rates between 1997 and 1998 in Beijing and Shanghai were similar.Serotyped by RT\|PCR method based on VP7 sequence,the positive rates of type G1,G2,G3 and G4 was 89\^5%,6\^1%,2\^8% and 0,respectively.Two cases were found being coinfected with G1 and G2,and one case was confirmed to be infected with G9 by cDNA sequence analysis.There was a little difference in prevalence of G type rotavirus infection among samples from different cities and different years.Conclusion The results indicated that rotavirus was the most important etiologic agent in this country,and the predominant serotypes were G1,G2 and G3.