喘康平冲剂抗病毒作用的实验研究

Experimental study of the virus inhibitory effect of Chuan-Kang-Ping Granule

目的 探讨喘康平冲剂的抗病毒作用。方法 在Hep 2细胞培养内观察细胞病变 ,测定喘康平冲剂对细胞的无毒限量 ,对呼吸道合胞病毒、柯萨奇病毒B3、流感病毒A1感染细胞病变的抑制指数 ,以及改变病毒和药物的加入顺序 ,观察细胞病变的影响程度。结果 喘康平冲剂的无毒限量是 1∶16 ,对呼吸道合胞病毒、柯萨奇病毒B3、流感病毒A1抑制指数为 8、8、2。双黄连口服液的无毒限量是 1∶32 ,对呼吸道合胞病毒、柯萨奇病毒B3、流感病毒A1的抑制指数为 8、16、2。改变病毒和药物的加入顺序 ,细胞病变程度不同。结论 喘康平冲剂对呼吸道合胞病毒 ,柯萨奇病毒B3、流感病毒A1确有抑制作用 ,与双黄连口服液一致或接近 ;其体外抗病毒作用机理主要是直接的灭污作用 。

Objective To explore the virus inhibitory effect of Chuan Kang Ping Granule. Methods\ Using tissue culture techniqe, the maximal non toxic concentration of the drugs in Hep 2 cell culture were measured and the virus inhibitory index of the drugs on the respiratory syncytial virus (RSV),the coxackie B 3 virus (Cox B 3)and the influenza A 1 virus (A 1) was calculated. In addition,the degrees of inhibition of the drugs on the cytopathic effect of viruses in cell culture was observed by changing sequence of adding the viruses and the drugs. Results The maximal non toxic concentration of Chuan\|Kang\|Ping Granule was 1∶16. The virus inhibitory index on RSV,CoxB 3,A 1 was 8,8 and 2, respectively The maximal non toxic concentration of Shuang Huang Lian oral administration Liquid was 1∶32. The virus inhibitory index on RSV,CoxB 3,A 1 was 8,16 and 2, respectively, The degrees of cytopathic effect were different when changing sequence of adding the viruses and the drugs. Conclusion Chuan Kang Ping Granule inhibited the replication of RSV,CoxB 3,A 1 in cultured cells. The inhibitory effects of the drugs were the same or nearly the same with Shuang\|Huang\|Lian oral administration liquid. The mechanism of the inhibitory effects of the drugs on the viruses was probably the direct killing effect in vitro, or the drugs might inhibit some key link of the replication of the viruses in cultured cells.