兔胫骨干骺端截骨延长延长区BMP、TGF-β_1的蛋白表达

Expression of BMP and TGF-β_1 in Regenerative Gap During Tibial Lengthening by Metaphyseal Osteotomy of Rabbits

目的 :研究肢体延长过程中延长区内源性BMP、TGF β1蛋白表达情况。方法 :采用兔胫骨干骺端截骨延长模型 ,通过免疫组化方法研究延长区内源性BMP、TGF β1蛋白表达情况。结果 :BMP、TGF β1主要表达在软骨细胞、成骨细胞、成纤维细胞中。BMP在开始延长后前 2周高度表达 ,2周后表达逐渐降低 ;停止延长 1周时在延长区中央见到少量的阳性表达细胞 ,停止延长 2周时 ,随着延长中央区域纤维组织消失 ,BMP阳性表达细胞也消失。TGF β1在延长前2周表达逐渐增强 ,到 2周时最强 ,2周后逐渐减弱 ,并在延长及停止延长期间均可见阳性表达细胞。结论 :肢体延长中延长区内源性BMP、TGF β1持续表达。

Objective: To study the expression of bone morphogetic protein(BMP) and transforming growth factor β 1(TGF β 1) in regenerative gap during lengthening period. Method: The right tibia of 14 mature rabbits were lengthened using metaphyseal osteotomy. Distraction was started on the 4th day postoperatively, 1mm per day and conducted twice each day. The expression of endogenesis BMP and TGF β 1 in regeneraive gap were observed during ten weeks after distraction by histology and immunohistochemisty. Results: The result showed that bone morphogetic protein(BMP) and transfoming growth factor β 1(TGF β 1) were expressed in chondrocytes, osteoblasts, and fibroblasts. The expression of BMP was at a high level in initial two weeks after distraction while the expression of TGF-β 1 was increased step by step. Then both of them decreased alonged with lengthening proceeding. Two weeks after distracting stopped, the expression of BMP could not being observed. But the expression of TGF-β 1 could being observed all stage after distraction stopped. Conclusion: BMP and TGF-β 1 were expressed persistently in regenerative gap during bone lengthening.