伤口巨噬细胞离子通道活动的实验研究

Experiments on ion channels in macrophages collected from wound

目的:研究伤口巨噬细胞离子通道活动的特点。方法:采用cell-attached膜片离子单通道记录方法。结果:伤口巨噬细胞膜上可记录到离子通道自发开放,这些自发性开放的通道包括以钾离子为载体的阳离子通道与以氯离子为载体的阴离子通道。文中对伤口巨噬细胞膜离子通道的生理功能进行了初步探讨。

Objective: To study the activities of ion channels in macrophages collected from wound. Methods:Using patch clamp technique to study the activities of ion channels.Results:The spontaneous occurrence of channel-induced currents in macrophage collected from wound can be recorded. These currents include cation-selective channel current carried by potassium and anion-selective channel current carried by chloridion. The physiologic function of these ion channels in macrophage collected from wound was discussed in this paper.